Affinity

Determine high-affinity interactions in-solution in about an hour: 

• K_D determination down to low picomolar levels
• No need to modify interactants
• Analyze affinities of up to 14 interacting pairs per CD 
• Set up in-solution affinity experiments guided by intuitive software

Measuring free fractions of interactants in equilibrated solutions with Gyrolab flow-through immunoassays provides unique advantages based on the short binding times for the affinity column. Short contact times of only a few seconds avoid the equilibrium shifts that are inherent in assays requiring long incubation times. 

Gyrolab Affinity Software module facilitates the design and evaluation of in-solution affinity determination experiments. The software guides you in defining the interacting pairs and setting up the affinity sample series. At equilibrium, the response from the unbound interactant in each affinity sample is measured with an automated Gyrolab Affinity Analysis run, requiring sample volumes as small as 4 µL.

Affinity analysis results are available for review immediately after the assay is completed.