The Kay lab at the University of Utah is focused on D-peptide inhibitor development, which requires the chemical synthesis of mirror-image protein targets. This webinar will describe the use of recently developed chemical and computational tools to accelerate chemical protein synthesis of large proteins. Specific topics will include recently developed next-generation “helping hand” traceless linkers to improve peptide solubility, the use of traceless templating to accelerate ligations, and our automated ligator program (Aligator) for prediction of the optimal synthetic routes.
LEARNING OBJECTIVES:
- “Helping hand” traceless linkers are a valuable tool for solubilizing difficult peptide segments.
- Templated ligations greatly accelerate ligations and can rescue otherwise suboptimal ligation junctions.
- Computational simulation of potential chemical protein synthesis routes can be used to predict optimal synthesis strategies.
