HEK 293 HCP Solution for Gyrolab^®

HEK 293 cells are commonly used for vector production of biopharmaceuticals, vaccines, and cell and gene therapies. The HEK 293 HCP Solution for Gyrolab consists of the HEK 293 HCP Assay Reagent Set for Gyrolab ordered from Cygnus Technologies and the Gyrolab Bioaffy™ 4000 HC Assay Toolbox.

HEK 293 HCP Solution for Gyrolab for the detection of HEK 293 host cell protein (HCP) impurities offers the following benefits:

• Automated workflows – reduced manual operations
• Broad dynamic range – over three logs
• Fast turnaround time – 96 data points in 95 minutes
• High throughput – up to 960 data points in a working day

The HEK 293 HCP Assay Reagent Set for Gyrolab is derived from the same antibodies and antigen used in the Cygnus 3^rd Generation Resupply HEK 293 HCP ELISA kit, (Cygnus Cat. no. F650S). The reagents are "generic” in the sense that they are intended to react with essentially all the HCPs that could contaminate the product independent of the purification process. 

This simple to use, high throughput, and semi-quantitative assay can be used to aid in optimal purification process development, process control, routine quality control, and product lot release. 

GRAPH: Gyrolab and ELISA assays of viral vector downstream bioprocessing samples show comparable results.